Figure 5.

Association of Calnexin and ERAD adaptor protein SEL1L with VLDLR WT and mutants: Cell lysates were prepared from transiently transfected cells expressing either wild-type or mutant VLDLR-HA (D487Y, D521H and C706F). Cell lysates (200 µg) were subjected to co-immunoprecipitation (Co-IP) using anti-HA agarose beads. Western blot analysis of the immunoprecipitated complexes were performed using antibodies against calnexin (a), SEL1L (b) and HA (c). Tubulin was used as a loading control for input (d) and IP beads probed against anti-mouse-HRP served as loading control for IP (d). The experiments were performed twice for each construct with identical results. Regions cropped from separate images are demarcated with borders. Unprocessed original scans of western blots are shown in Supplementary Figure S12.