Figure 7.

Exogenous expression of SEL1L enhances the degradation of VLDLR WT and mutant C706F in SEL1L Knockout cell lines: (a) HEK-293 and SEL1L K/O cells were transfected with VLDLR-WT plasmid alone or co-transfected with VLDLR-WT and SEL1L constructs. At 24 h post-transfection, the cells were treated with 100 µg/ml CHX (24 C) or DMSO (24D) for 24 h and cells were harvested for western blot analysis. Total cell lysates were analysed by immunoblotting against antibodies for HA, tubulin and SEL1L. (b) Graph representing densitometric analysis of 6 independent experiments conducted in knock-out cells generated by different gRNAs. (*) p ≤ 0.05; (***) p ≤ 0.001; Two way ANOVA. (c) Cells expressing VLDLR mutant C706F alone or SEL1L as described in (a) were treated with CHX for 24 h and western blots were generated as in (a). (d) Densitometric analysis of six independent experiments from knock-out cell lines generated by different gRNAs, (*) p ≤ 0.05; (**); p ≤ 0.01; (***) p ≤ 0.001; Two way ANOVA, Bonferroni post-test. Regions cropped from separate images are demarcated with borders. Unprocessed original scans of western blots are shown in Supplementary Figure S13.