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. 2018 Jan 25;8:1605. doi: 10.1038/s41598-018-19407-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PD-L1 fusion protein treatment regulates T cell enrichment. (A) MPM were isolated from Pmel-1 mice. PD-L1 were detected by flow cytometry with specific anti-PD-L1 antibody. (B) Quantification of PD-L1 expression in MPM from (A). *p < 0.05. (C) Expression of FoxP3 and CD3 was evaluated by immunofluorescence staining with specific anti-FoxP3 and anti-CD3 antibodies and then quantified. (D) Splenocytes were isolated and assayed for Treg cell population by flow cytometry. Representative flow cytometry images of FoxP3⁺CD3⁺ cell population were showed (Control group n = 6 cases, PD-L1 fusion protein treated group n = 6 cases). (E) Quantification of Treg cells population from (C) were shown. *p < 0.05.