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. 2018 Jan 25;9:368. doi: 10.1038/s41467-017-02796-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Deletion of Ino80 using sinus venosus- or endocardial-specific Cre lines. a Schematic section through the heart showing the two types of endothelial cells within the heart and the Cre lines that differentially label each subset. b Endomucin (endocardial cells) and DAPI (nuclei) immunofluorescence in e15.5 hearts highlights reduction of compact myocardial thickness in hearts where Ino80 was deleted using either Nfatc1Cre, ApjCreER, or Tie2Cre. Images are representative of the following number of replicates: Nfatc1Cre (control, n = 9 hearts; mutant, n = 6 hearts), ApjCreER (control, n = 12 hearts; mutant, n = 4 hearts), or Tie2Cre (control, n = 9 hearts; mutant, n = 6 hearts). Conditional knockouts are labeled CKO. Scale bars: 100 μm. c Quantification of compact and trabecular myocardial thickness in each condition. Error bars in graphs are standard deviation. Nfatc1Cre (control, n = 9 hearts; mutant, n = 6 hearts), ApjCreER (control, n = 12 hearts; mutant, n = 4 hearts), or Tie2Cre (control, n = 9 hearts; mutant, n = 6 hearts). *P < 0.05; **P < 0.01; ***P < 0.001, evaluated by Student’s t-test. d Example of abnormal extensions of Endomucin-positive endocardial cells (arrowheads) into the compact layer in CKO embryos. H&E sections are of comparable regions of the apex. Images are representative of the following number of replicates: Nfatc1Cre (control, n = 9 hearts; mutant, n = 6 hearts), ApjCreER (control, n = 12 hearts; mutant, n = 4 hearts). Scale bars: 100 μm