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. 2018 Jan 25;8:1568. doi: 10.1038/s41598-018-19834-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stability ranking of candidate reference genes by geNorm in the first stage of the study. (A) Gene expression stability (geNorm M) in all samples. Least stable to the left and most stable to the right. (B) Determination of the optimal number of reference genes. The V_2/3 value was below the 0.15 threshold and the optimal number of reference genes was two. (C) Gene expression stability (geNorm M) in subgroups.