Fig. 2.

Lsd1 overexpression accelerates skeletal muscle regeneration. a Tibialis muscle mass normalized to body mass of control mice (Ctrl) or mice overexpressing LSD1 selectively in Myf5-positive muscle precursors (LSD1^cTg) 5 days after Ctx or vehicle injection (left panel). Number of regenerating centronuclear fibers per area (mm²) in Ctrl and LSD1^cTg mice 5 days after Ctx treatment (right panel). Significance was calculated by two-way ANOVA test. b Gomori staining of representative tibialis muscle sections from Ctrl and LSD1^cTg mice 0, 5, and 7 days after Ctx treatment. c CSA measurement of regenerating centronuclear muscle fibers from Ctrl and LSD1^cTg mice 5 and 7 days after Ctx treatment. Significance was calculated by two-way ANOVA test. d Immunofluorescence assay using antibodies directed against pan-myosin heavy chain (Myh, red) and green fluorescent protein (GFP, green) on control (Ctrl-GFP) and LSD1 overexpressing (LSD1-GFP) satellite cells isolated from extensor digitorum longus (EDL) fiber culture differentiated in myogenic medium for 0, 1, 3, or 7 days. Nuclei were stained with DAPI (blue). e Quantification of the nuclei per fiber shown in immunofluorescence assay in d. Significance was calculated by two-tailed Student’s t-test. (a, c: n = 5, e: n = 3; mean + SEM, NS = non-significant, *p < 0.05, **p < 0.01, ***p < 0.001; scale bars: b, d 100 µm)