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. 2018 Feb;188(2):343–352. doi: 10.1016/j.ajpath.2017.10.012

Figure 1.

Figure 1

Inducible degrader of the low-density lipoprotein (LDL) receptor (IDOL) overexpression results in elevated cholesterol levels. Human degradation–resistant IDOL was expressed using a liver-specific adenoassociated virus (AAV) serotype DJ/8 [or enhanced green fluorescent protein (eGFP)-AAV-DJ/8 as a control) in 5-week–old male black and tan, brachyury (BTBR) wild-type (WT) and BTBR leptin-deficient (OB) mice. The mice were then fed a high-fat diet for 18 weeks. A: Body weight throughout the study. B: Blood glucose. C: Blood cholesterol. D: Plasma triglycerides (TG). E: Liver MYLIP mRNA using primers that detect both mouse and human MYLIP (gene encoding IDOL). F: Liver LDL receptor levels determined by Western blot and normalized to glyceraldehyde-3-phosphate dehydrogenase. G: Cholesterol lipoprotein profiles. H: Quantification of cholesterol in each lipoprotein based on G. Data are expressed as means ± SEM. n = 9 to 15 (A–D, H); n = 5 to 9 (E); n = 4 to 7 (F); n = 3 to 4 (G). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 compared with the WT eGFP group; †P < 0.05 compared with the OB eGFP group (two-way analysis of variance). HDL, high-density lipoprotein; VLDL, very low-density lipoprotein.