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. 2017 Nov 9;9(6):1961–1975. doi: 10.1016/j.stemcr.2017.10.004

Figure 2.

Figure 2

Loss of RhoA Disrupts Epithelial Polarity and Adhesion, while Reducing Proliferation and Inducing Apoptosis in Small Intestine

(A) qRT-PCR analysis showing gene expression of cell junction and polarity markers in control WT and RhoA KO small intestine. Data are normalized to GAPDH expression; n = 3 mice per genotype. Error bars represent SD from three independent experiments.

(B–D) Representative immunofluorescence staining of Na+,K+-ATPase α (B), phalloidin (C), and Ki67 (D) in control WT and RhoA KO duodenum.

(E) Quantification of cleaved caspase-3-positive cells per crypt in control WT and RhoA KO duodenum. n = 4 mice for each genotype. Error bars represent SD from three independent experiments.

(F) Western blotting of apoptotic related proteins Bcl-2, Bcl-xL, p53, p-P53, and cleaved caspase-3 in duodenal crypts isolated from control and RhoA KO mice.

∗∗p < 0.01, ∗∗∗p < 0.001.