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. 2017 Nov 9;9(6):2081–2096. doi: 10.1016/j.stemcr.2017.10.009

Figure 7.

Figure 7

Effect of Ground-State-Associated miRNAs on ESC Differentiation

(A) qRT-PCR analysis of miRNAs 7 days after induction of EB formation. snoRNA202 was used as an internal normalization control. Data are shown as mean ± SD, n = 3. ∗p < 0.0001.

(B) qRT-PCR analysis of transcripts associated with pluripotency and differentiation 7 days following serum ESC treatment with miRNAs in the absence of LIF. Gapdh was used as an internal normalization control. Data are shown as mean ± SD, n = 3. ∗p < 0.05.

(C) Percentages of AP-positive and -negative EBs 7 days after transfection of ESCs in the absence of LIF.

(D) AP staining of EBs treated with miRNAs 7 days post differentiation. Scale bar, 200 μm.

(E) qRT-PCR analysis of gene transcripts associated with pluripotency and differentiation 7 days following 2i ESC treatment with miRNA inhibitors in the absence of 2i/LIF. Gapdh was used as an internal normalization control. Data are shown as mean ± SD, n = 3. ∗p < 0.05.