Figure 4.

AD Astrocytes Show Altered Cytokine Release in Pro-inflammatory Conditions

Concentrations of IL-2, IL-6, IL-10, GM-CSF, and CCL5 were quantified from media after stimulation with TNFα (50 ng/mL) and IL-1β (10 ng/mL) for 48 hr with CBA assay. Results are shown as fold change to control lines. DAPT: cells were treated with γ-secretase inhibitor DAPT simultaneously with TNFα and IL-1β stimulation. Data are presented as mean ± SEM from three independent experiments (CTRL, n = 6 lines; AD, n = 6 lines; ^∗∗p < 0.01, ^∗∗∗p < 0.001). See also Figure S3.