Figure 3.

Functional Characterizations of hiHeps and iPSC-HLCs

(A) Glycogen storage in different HLCs was measured by periodic acid-Schiff (PAS) staining. Scale bar, 100 μm.

(B) Glycogen storage in HLCs was determined quantitatively by colorimetric measurement (Abnova). UCF included two independent replicates, UCF1 and UCF2; hiHep included four replicates from independent experiments (hiHep1, hiHep2, hiHep3, and hiHep4); iPSC-HLC included four replicates from independent experiments (iPSC-HLC1, iPSC-HLC2, iPSC-HLC3, and iPSC-HLC4). PHH included two replicates cultured for 2 days from independent experiments.

(C) HLCs both eliminated testosterone as efficiently as PHHs. Concentrations of testosterone were determined by liquid chromatography-tandem mass spectrometry. Each time point had three replicates from independent experiments.

(D) iPSC-HLCs showed more lipid accumulation than hiHep as measured by oil red O staining. Scale bar, 100 μm.

(E) Lipid accumulation was quantified by oil lipid numbers per cell. UCF had two independent replicates, UCF1 and UCF2; hiHep had four replicates from independent experiments (hiHep1, hiHep2, hiHep3, and hiHep4); iPSC-HLC had four replicates from independent experiments (iPSC-HLC1, iPSC-HLC2, iPSC-HLC3, and iPSC-HLC4). PHH included two replicates cultured for 2 days from independent experiments. There was a significant difference between hiHep and iPSC-HLC. ^∗p < 0.05.

(F) UGT activities of hiHeps and iPSC-HLCs were determined by the luminescence of remaining substrates. The combination of replicates is the same as in (E). There was a significant difference between hiHep and iPSC-HLC. ^∗p < 0.05.

See also Figure S3.