Figure 5.

Expansion of Fresh and Cryopreserved PBC-iPSC-NK Cells

(A and B) Expansion of fresh PBC-iPSC#9-NK cells by K562-mbIL15-41BBL in G-Rex10 starting with different NK cell numbers. Absolute numbers of NK cells during a 14-day expansion (A) and fold changes after expansion (B) are shown.

(C and D) Phenotype (C) and cytotoxicity against K562 (D) of fresh PBC-iPSC#9-NK cells after expansion as measured by flow cytometry.

(E) Viability of expanded PBC-iPSC#9-NK cells after freeze/thaw procedure.

(F) Expansion of cryopreserved PBC-iPSC#9-NK cells.

(G) Cytotoxicity of cryopreserved PBC-iPSC#9-NK cells against K562 after expansion. These data are representative of three independent experiments.