Figure 7.

KIR Typing of PBC-iPSC-NK Cells

(A–J) KIR genotyping and mRNA expression profiling. Electrophoresis of PCR products of genomic DNA shows KIR gene content and endogenous β-actin gene of a PB-NK donor (donor 4) (A) and three PBC donors for PBC-iPSC#9 (donor A) (C), PBC1-iPSC#4 (donor B) (G), and PBC2-iPSC#12 (donor C) (I). Electrophoresis of PCR products of cDNA shows mRNA expression of KIR genes and endogenous β-actin gene in PB-NK cells (B), PBC-iPSC#9 cells (D), PBC-iPSC#9-NK cells (E), post-expansion PBC-iPSC#9-NK cells (F), PBC1-iPSC#4-NK cells (H), and PBC2-iPSC#12-NK cells (J).

(K–P) KIR phenotyping of different NK cells by flow cytometry.