Figure 1.

CbpS436A Enhances the Reprogramming Efficiency of i-Pericytes to NPCs in Culture

(A) Schematic of experimental flowchart.

(B) Images of i-pericytes cultured for 7 days, showing PDGFRβ+ (red)/NG2+ (green)/SOX2− (purple) cells. Scale bar, 25 μm.

(C) A neurosphere reprogrammed from i-pericytes expressed Sox2 (red) after cytospin. Scale bar, 10 μm.

(D) Differentiation of neurospheres that were reprogrammed from i-pericytes into βIII TUBULIN+ neurons (left panel), GFAP+ astrocytes (middle panel), and O4+ oligodendrocytes (right panel) in the presence of retinoic acid. Arrows denote positive cells for βIII TUBULIN, GFAP, or O4. Scale bar, 20 μm.

(E and F) Images (E) and quantitative analysis (F) of the number of neurospheres reprogrammed from i-pericytes isolated from wild-type (WT) and CbpS436A-KI stroke tissues. Scale bar, 150 μm. ^∗p ≤ 0.05, n = 4/group.

(G) Quantitative analysis of the number of neurospheres reprogrammed from i-pericytes in the absence and presence of compound C (1 μM). ^∗p ≤ 0.05, n = 4/group.

Error bars in this figure represent the SEM.