Figure 2.

Organotypic-like Coculture Recapitulates Human Astrocyte Complexity

(A) Image of cultured tissue (yellow arrow) in a well of a six-well plate on a PTFE membrane insert.

(B) Comparison of organoid spheres plated on either Matrigel or PTFE, with or without NGN2 induction, demonstrating the presence or absence of rosette structures, respectively, at the same image magnification.

(C) (Left) Merge of bright-field and GFP fluorescence of mGFP astrospheres plated on top of organotypic-like iNeurons. (Middle) A single-plane confocal image of hAstros after 4 weeks of coculture. (Right) Flattened z series of a single hAstro.

(D) Time series representing the dynamic morphological complexity of cocultured hAstros.

Scale bars, 50 μm.