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Bioengineering and Assembly of 3D Spheres
(A) Bioreactor spinner flasks enable long-term separation of spheres, whereas close physical contact promotes merging of multiple spheres.
(B) Morphologically complex hAstros are evenly, yet seemingly randomly, dispersed throughout the asteroids. Neurons are MAP2 positive.
(C and D) Examples of 3D imaging demonstrate the macroscale composition of integrated spheres.
