Figure 3.

M⁺ COUP-red CMs Exhibit Atrial Properties

(A) Analyzed AP parameters.

(B) Representative AP of G⁺/M⁺ CMs generated from RA-treated and G⁺/M⁻ CMs from CT differentiations at 1 Hz stimulation.

(C) Averaged maximum diastolic potential (MDP), maximum AP amplitude (APA_max) and AP plateau amplitude (APA_plat), maximum upstroke velocity (dV/dt_max), and AP duration at 20%, 50%, and 90% repolarization (APD₂₀, APD₅₀, and APD₉₀). Scatterplot depicting the APA_plat of single CMs and calculated median from RA and CT conditions. n = 21 cells for RA G⁺/M⁺ and n = 18 cells for CT G⁺/M⁻; from 3 independent differentiations. Data are displayed as means ± SEM; ^∗p < 0.05.

(D) Typical AP traces stimulated at different frequencies.

(E) Transcriptional profiling of selected atrial or ventricular-specific genes by qPCR of G⁺/M⁺ and G⁻/M⁺ fractions from RA differentiations compared with G⁺/M⁻ and G⁻/M⁻ fractions from CT differentiations at D20 of differentiations (n = 4). Data are displayed as means ± SEM; ^∗p < 0.05.

(F) Genome-wide transcriptional profiling by microarray. Heatmaps to display the fold difference of selected atrial- and ventricular-enriched transcripts in RA-treated G⁺/M⁺ and CT G⁺/M⁻ from COUP-red at a threshold of 2-fold difference.

See also Figure S3.