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. 2018 Jan 19;8:2011. doi: 10.3389/fimmu.2017.02011

Table 1.

Primers used for quantification of viral mRNA, IFN-stimulated genes, chemokines, and cytokines.

Primer name Sequence (5′–3′) Use
Retinoic acid-inducible gene I (RIG-I) F GCGTCTCAGTGCAGCACATCATT qRT-PCR
RIG-I R GGGTCCCGTGACTCTCCAAGTTT qRT-PCR
Melanoma differentiation-associated protein 5 (MDA5) F GCTGCTAAAGACGGAAATCG qRT-PCR
MDA5 R CTTGTCGCTGTCATTGAGGA qRT-PCR
Mitochondrial antiviral-signaling protein (MAVS) F ATGCCGTTTGCTGAAGAC qRT-PCR
MAVS R CTAGTGCAGACGCCGCCG qRT-PCR
IRF7 F CTGGAGCCATGGGTATGCA qRT-PCR
IRF7 R AAGCACAAGCCGAGACTGCT qRT-PCR
Interferon (IFN)-β F CACAGCCCTCTCCATCAAC qRT-PCR
IFN-β R GCATCTTCTCCGTCATCTCC qRT-PCR
STAT1 F GTGGACATTGAGTTCTTGGTGAGATCC qRT-PCR
STAT1 R CCTTTCTCATTCTGTCGACTTTGTTGG qRT-PCR
IFIT1 F GCATCACCTTCCTCTGGCTA qRT-PCR
IFIT1 R TGTTGTTCAGTGCCTTCTGG qRT-PCR
Rabies virus (RABV) NF ACACCGGCAACTACAAGACA qRT-PCR and cDNA synthesis
RABV NR ATGGTACTCCAGTTGGCACA qRT-PCR
β-actin F AGGTGACAGCATTGCTTCTG qRT-PCR
β-actin R GCTGCCTCAACACCTCAAC qRT-PCR