Figure 5.

APLN, OSM, and POSTN Loss-of-Function Experiment on Myogenesis/Angiogenesis Coupling

The 3D assay used EC-GFP and RFP-MPCs that were embedded in the gel together or alone. In some experiments, IgGs, APLN receptor inhibitor ML221 (16 μM), anti-OSM, or anti-POSTN blocking antibodies (50 and 40 μg/mL) were added. Six days later, capillary length (A), lumenization (C), and myotube fusion (E) were evaluated. On the right, pictures present examples of capillary elongation (B), capillary lumenization (green, arrows) (D), and myotube formation (red, arrowheads) (F) in EC + MPC conditions with or without the inhibitors. Results are means ± SEM of at least three independent experiments. Mann-Whitney test was performed versus EC + MPC: ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; and versus MPC + IgG: $p < 0.05; $$p < 0.01. Bars, 100 μm. See also Figure S3.