Figure 6.

Effect of Macrophages on Myogenesis/Angiogenesis Coupling

(A–C) Expression of Apln (A), Osm (B), and Postn (C) by ECs, SCs, macrophages (MP), and FAPs at day 2 and 4 of regeneration, as described in Figure S4.

(D, E, G, and I) 3D assay was performed with macrophages previously activated with interleukin (IL)-4 or IL-10/dexamethasone (DEX) layered on top of the gel (D). Six days later, capillary length (E), lumenization (G), and myotube formation (I) were quantified. In some experiments, anti-OSM blocking antibodies (50 μg/mL) were added.

(F and H) Representative pictures with capillary lumen formation (green, arrows) and myotube formation (red, arrowheads).

Results are means ± SEM of four to six independent experiments. Mann-Whitney test was performed versus none: ^∗p < 0.05; and versus Mp + IL10: $p < 0.05. Bars, 100 μm.