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. 2017 Nov 30;9(6):1853–1867. doi: 10.1016/j.stemcr.2017.10.029

Figure 2.

Figure 2

Defective Differentiation of Neuronopathic GD NPCs to DA Neurons

(A) Representative confocal z stack images from control, GD2, and GD3 dopaminergic neuronal cultures differentiated as described in the legend to Figure 1A. Neurons were co-labeled with anti-TUJ1 (red) and anti-TH (green) antibodies. Magnification 40×; scale bar, 50 μm.

(B) Quantitative analysis of TH expression in control, GD2, and GD3 DA neurons. Bar graph represents the percentage of TH-positive neurons relative to control counted in three different fields per experiment. n = 5 per group ± SEM (compiled data are repeats from two GD2 and two GD3 patients). ∗∗p < 0.005 between control and both GD2 and GD3 as assessed by one-way ANOVA.

(C) Representative immunofluorescence images for DA marker expression in control and GD2 DA neuronal cultures. Neurons were co-stained with antibodies to TUJ1 (red) and antibodies to PITX1, VMAT2, LMX1, GIRK2, or FOX2A (green) as indicated. Magnification 20×; scale bar, 100 μm.