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. 2017 Nov 30;9(6):1853–1867. doi: 10.1016/j.stemcr.2017.10.029

Figure 5.

Figure 5

Decreased Survival of Neuronopathic GD NPCs in the Absence of Exogenous Wnt Activation

(A) Schematic representation of the protocol used for directed differentiation of iPSCs to DA neurons as described by Kriks et al. (2011).

(B) Representative immunofluorescence images for TH expression (green) in control and GD2 DA neurons generated by directed differentiation of iPSCs as shown in (A). Nuclei were labeled with DAPI (blue). Magnification 20×; scale bar, 100 μm.

(C) Control and GD iPSC were differentiated in the presence or absence of CHIR as indicated in the text. Images show the culture dishes containing control and GD2 iPSC cultures at day 13 of differentiation following the protocol described in (A).

(D) Control and GD2 iPSCs were differentiated in the presence or absence of CHIR and rGCase as indicated in the text. Panels show representative phase-contrast images for control and GD2 iPSC cultures at day 13 of DA differentiation following the protocol described in (A). Insets are an enlargement of a small area in each condition. Magnification 10×; scale bar, 100 μm.