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. 2017 Nov 9;118(2):233–247. doi: 10.1038/bjc.2017.385

Figure 6.

Figure 6

BPIFB1 inhibits VIM-induced migration and invasion of NPC cells. (A) BPIFB1 and VIM colocalisation in 5-8F cells. DAPI-stained nuclei: blue; anti-Flag-BPIFB1: red; anti-His-VIM: green; merged image represents the overlay of DAPI, Flag, and His signals; BPIFB1 and VIM colocalisation: yellow. Scatter analysis shows the signals of channel 561 (BPIFB1) and 488 (VIM). Pearson’s correlation coefficient of colocalisation is indicated in the top right corner of the plot. Scale bar=29 μm. (B) BPIFB1 and VIM expression was confirmed by western blot in 5-8F cells transfected or co-transfected with BPIFB1-Flag and VIM-His vectors using anti-Flag and anti-His primary antibodies. (C and D) Representative images of migration and transwell Matrigel invasion assays of 5-8F cells transfected or co-transfected with BPIFB1-Flag and VIM-His vectors. Data represent the mean±s.d. and are representative of three independent experiments. *P<0.05; **P<0.01; ***P<0.001; n.s., no significance.