Skip to main content
. 2017 Dec 20;4(1):71–80. doi: 10.1021/acscentsci.7b00440

Figure 3.

Figure 3

KRas4b interacts with eIF2Bδ through its C-terminal HVR. (a) Heavy/light ratio of eIF2Bδ in KRas4a/b SILAC and the primary mass spectra of one eIF2Bδ peptide (residues 210–225) in forward and reverse KRas4b SILAC. (b) Immunoprecipitation of FLAG-tagged KRas4b, but not KRas4a, pulled out endogenous eIF2Bδ in HEK293T cells. (c) Immunoprecipitation of FLAG-tagged HRas(1–164)-KRas4b (165–188), but not HRas(1–164)-KRas4a (165–189), pulled out endogenous eIF2Bδ as FLAG-tagged KRas4b did. (d) Immunoprecipitation of FLAG-tagged KRas4b WT, G12D and S17N pulled out similar levels of endogenous eIF2Bδ. (e) Heavy/light ratios of eIF2Bα, eIF2Bβ, eIF2Bγ, and eIF2Bε in KRas4a/b SILAC.