Fig. 1.

Dose- and time-dependent effect of leptin on BC cell viability. a Dose-Dependent effect- ~ 60–70% confluent MCF-7, ZR-75-1 and MDA-MB-231 cells were grown in serum-deprived DMEM for 96 h in the presence or absence of different doses of Leptin and cell viability was measured using Crystal Violet assay. The data represents mean ± SEM of eight independent experiments. b Time-dependent effect- ~ 60–70% confluent MCF-7, ZR-75-1 and MDA-MB-231 cells were grown in serum-deprived DMEM for different times (i.e., 24 h, 48 h, 72 h and 96 h) in the presence or absence of Leptin (3.125 nM) and cell viability was measured using Crystal Violet assay. The data represents mean ± SEM of eight independent experiments