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. 2018 Jan 26;8:1679. doi: 10.1038/s41598-018-19828-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Gbp1 downregulation on mitophagy in macrophages. (a) Autophagic proteins p62 and LC3I/II levels in macrophages treated with LPS (1 μg/ml) or IFNγ (10 ng/ml) for 1 hour under fed or starvation conditions. (b) Autophagic protein levels in mitochondrial fraction of macrophages treated with LPS (100 ng/ml) or IFNγ (10 ng/ml) in the presence or absence BafA1 (100 nM) for 4 hours. (c) The levels of mitophagy related proteins in cytosolic and mitochondrial fractions isolated from macrophages treated with 100 ng/ml LPS or 10 μM CCCP for 4 hours. (d,e) mRNA expression levels of genes encoding anti-oxidative enzymes in macrophages treated with either 100 ng/ml LPS (d) or 10 ng/ml IFNγ (e) for 24 hours. The results are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 versus Scr cells. HBSS: Hanks’ Balanced Salt Solution; CCCP: Carbonyl cyanide m-chlorophenylhydrazone. Full-length of all blots are provided in Supplementary Fig. 8.