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. 2018 Jan 26;8:1679. doi: 10.1038/s41598-018-19828-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Gbp1 downregulation on LPS-induced glycolysis in macrophages. (a–d) ECAR in Scr and Gbp1 Kd macrophages treated with LPS (100 ng/ml) for 4 hours (n = 5). Back.Corr.: Background Correction. (e) 2-Deoxy glucose uptake in Scr and Gbp1 Kd macrophages under basal and LPS-treated conditions. Three independent experiments were performed. Error bars represent standard error. (f,g) mRNA expression levels of genes involved in glucose uptake and glycogen synthesis in Scr and Gbp1 Kd macrophages. The data for gene expression are presented as mean ± SEM; the experiment was repeated 2–3 times. *P < 0.05, **P < 0.01, ***P < 0.001; versus Scr cells.