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. 2018 Jan 26;8:1637. doi: 10.1038/s41598-018-20022-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CRM and CLSM imaging of alginate microbeads with heterogeneous (a–d) and homogeneous (e–h) spatial distribution of alginate. (a,e) CLSM image in the transmission mode. (b,f) CLSM image in the fluorescence emission mode at the equatorial microbead cross-section. (c,g) Overlay of CLSM and CRM intensity profiles at the equatorial microbead cross-section. (d,h) Spatial distribution of alginate in the microbeads shown in (c) and (g), respectively, expressed as the absolute alginate concentration in wt.%. Bars in (a,b,e,f) are equal to 100 μm.