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. 2018 Jan 26;8:1638. doi: 10.1038/s41598-018-20095-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neuronal apoptosis of ischemic cortex in MCAO rats undergoing GFR and RFR intervention. (A) Representative photomicrographs of TUNEL-positive cells (brown staining) in the MCA territory of the ischemic cortex (magnification 400×) after 15, 30 and 60 min MCAO of rats in GFR and RFR intervention. Scale bar = 100 μm. (B) Comparison of neuronal apoptosis of rats following GFR and RFR treatment after 15-, 30- and 60-minute MCAO. The TUNEL-positive cells were quantified by NuclearQuant module software (3D, HISTECH Ltd., Budapest, Hungary) and represented as dot plot of the raw data, overlaid by box and whisker plot (median, first and third percentile, range). Significance was determined by independent Student’s t test. *p < 0.05 and **p < 0.01 means statistical significance in GFR group versus RFR group.