Figure 2.

NLS-4λN-DD and 4λN-DD E488Q are localized in the nucleus. (A) Schematic of our editing enzymes (4λN-DD WT and E488Q) with and without the NLS fused to the N-terminus. (B) Immunostaining of both NLS and non-NLS versions of our editing enzymes transiently transfected in HEK293T cells visualized with a Nikon A1R Confocal Laser Microscope.100X images are shown and scale bars = 19μm; n = 2. (C) Western blot analysis of expression of 4λN-DD WT and E488Q with and without NLS in total, cytoplasmic and nuclear fractions extracted from HEK293T cells. 4λN-DD WT and E488Q are 60 kDa while both enzymes with the appended NLS are 64 kDa. Tubulin is 50 kDa and histone is 18 kDa; n = 2.