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. 2018 Jan 26;8:1660. doi: 10.1038/s41598-018-20145-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of full-length and truncated GFP-PrkC in B. subtilis. 3D representation of GFP-PrkC fusion proteins, with A to G letters and dashed lines indicating the length of the protein. PrkC molecular graphic was modeled with the UCSF Chimera package (supported by NIGMS P41-GM103311) from the 3PY9 PDB structure for the extracellular domain and 4EQM PDB structure for the intracellular domain. Strains were grown on LB medium at 37 °C and all the forms of GFP-PrkC proteins were expressed from the P_xyl promoter in the presence of 0.5% xylose and with 3 µg/ml of PG fragments for the full-length protein. PrkC localization was analyzed by fluorescent microscopy for strains: A: SG278 (ΔprkC, amyE::gfp-prkC), A’: SG278 in the presence of PG fragments, B: SG467 (ΔprkC, amyE::gfp-prkCc-TM-P1P2), C: SG466 (ΔprkC, amyE::gfp-prkCc-TM-P1), D: SG465 (ΔprkC, amyE::gfp-prkCc-TM), E: SG355 (ΔprkC, amyE::gfp-prkCc), F: SG497 (ΔprkC, amyE::gfp-TM-P1P2-Ig-like) and G: SG498 (ΔprkC, amyE::gfp-TM). The scale bar for microscopy images represents 2 µm.