Table 1.
B. subtilis strains and plasmids used in this work.
| Strain/plasmid Relevant characteristics | Source/reference | |
|---|---|---|
| B. subtilis strains | ||
| WT168 | trpC2 | lab collection |
| 1A963 | trpC2 prkC∆1 | 17 |
| SG278 | trpC2 prkC∆1, amyE::gfp-prkC | 20 |
| SG355 | trpC2 prkC∆1, amyE::gfp-prkCc | This work |
| SG465 | trpC2 prkC∆1, amyE::gfp-prkCc-TM | This work |
| SG466 | trpC2 prkC∆1, amyE::gfp-prkCc-TM-P1 | This work |
| SG467 | trpC2 prkC∆1, amyE::gfp-prkCc-TM-P1P2 | This work |
| SG497 | trpC2 prkC∆1, amyE::gfp-TM-P1P2P3-Ig-like | This work |
| SG498 | trpC2 prkC∆1, amyE::gfp-TM | This work |
| SG622 | trpC2 prkC∆1, amyE::gfp-prkC(R500A) | This work |
| Plasmids | ||
| psG1729-prkCc | This work | |
| psG1729-prkCc-TM | This work | |
| psG1729-prkCc-TM-P1 | This work | |
| psG1729-prkCc-TM-P1P2 | This work | |
| psG1729-TM-P1P2P3-Ig-like | This work | |
| psG1729-TM | This work | |
| psG1729-prkC(R500A) | This work | |
| pETDuet-prkC | This work | |
| pETDuet-prkC-TM | This work | |
| pETDuet-prkC-TM-P1 | This work | |
| pETDuet-prkC-TM-P1P2 | This work | |
| pETDuet-prkC(R500A) | This work | |