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. 2018 Jan 26;8:1655. doi: 10.1038/s41598-018-19974-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GeneScan results of partial TCR AV and BV gene families in CD4⁺ T cells from healthy control-1 (C1) and C-strain vaccine immunised pig-4 (H4). (a) The polymerase chain reaction (PCR) products of TCR DV1-AV5S of C1 analyzed on 1.5% agarose gel stained with ethidium bromide. (b) PCR products of TCR BV2S-BV6S of C1 analyzed on 1.5% agarose gel stained with ethidium bromide. (c) Partial fluorescence-labeled PCR products of C1 analyzed on 6% acrylamide sequencing gel. (d) Partial fluorescence-labeled PCR products of H4 analyzed on 6% acrylamide sequencing gel. (e) CDR3 size and fluorescence intensity analysis of TCR AV8-4S and AV13 S of C1. (f) CDR3 size and fluorescence intensity analysis of TCR AV14S and AV5S of H4.