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. 2018 Jan 26;8:1655. doi: 10.1038/s41598-018-19974-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Clonal expansions of TCR AV and BV gene families in CD4⁺ T cells from pigs immunised with C-strain vaccine. Vα/β families with clonal expansions are depicted for post immunisation (left column) and before immunisation (recognized as control, right column). In each column, CDR3 size profiles and fluorescence intensity of selected families are shown on the left and RI value for each peak on the right. Numbers from −5 to +5 correspond to normally distributed CDR3 profiles from pigs before immunisation, where 0 is the major central peak. Data are shown as RI, calculated as each peak are against the total peak area. (a) TCR AV gene families showing evidence of clonal expansions. (b) TCR BV gene families showing evidence of clonal expansions.