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. 2018 Jan 26;8:1655. doi: 10.1038/s41598-018-19974-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the TRAV/BV CDR3 size selection and TRAJ/BJ gene usage by CD4⁺ T cells from C-strain vaccine immunised pigs. PCR products of CDR3 from clonally expanded TCR-α and -β chains were cloned and sequenced. (a) CDR3 size preferences by all sequenced Vα and Vβ genes from CD4⁺ T cells of C-strain immunised pigs are presented as percentage; 80% of clonally expanded T cells had a α CDR3 region of 9–11 aa in length, and preferred size was 29%; 68% of clonally expanded T cells had a β CDR3 region of 11–13, and preferred size was 33%. (b) The usage of TRAJ/BJ genes from clonally expanded TCR-α and -β chains in CD4⁺ T cells from pigs immunised by C-strain CSFV vaccine. “?” refers to the sequence that we didn’t find the corresponding TRBJ gene on GeneBank CoreNucleotide database (https://www.ncbi.nlm.nih.gov/genbank/) or IMGT website (http://www.imgt.org/).