Table 2.
The CDR3 amino acid sequences of clonal TCR-α chain in CD4+ T cells in three Hezuo miniature pigs immunised with C-strain vaccine.
| Case | TCR AV gene family | Peak | Size (aa) | 3′ Vα | N + 5′ proximal Jα | Jα | Frequency |
|---|---|---|---|---|---|---|---|
| H2 | AV14 | 0 | 11 | CALS | ERDTRTDKLTFGDG | Jα27 | 3/14 (21.4%) |
| 11 | CALS | ERRLSYDKLIFGSG | Jα34 | 2/14 (14.3%) | |||
| 11 | CALS | ESRQTGYRLVFGKG | Jα15 | 2/14 (14.3%) | |||
| Others | 7/14 (50%) | ||||||
| AV38 | 0 | 9 | CAYS | ASNNNDLRFGAG | Jα43 | 3/11 (27.3%) | |
| 9 | CAYS | ATNNNDLRFGAG | Jα43 | 2/11 (18.2%) | |||
| 11 | CAYS | ARGGSNYKLTFGKG | Jα53 | 2/11 (18.2%) | |||
| 10 | CAYS | AQGGAAGVFFGKG | Jα57 | 2/11 (18.2%) | |||
| Others | 2/11 (18.2%) | ||||||
| H3 | AV8-3S | 0 | 8 | CAVS | FRNNDLRFGAG | Jα43 | 3/10 (30%) |
| 10 | CAVS | GNNGGFKGVFGAG | Jα9 | 2/10 (20%) | |||
| 9 | CAVS | ADGGYKWIFGRG | Jα12 | 2/10 (20%) | |||
| Others | 3/10 (30%) | ||||||
| H4 | AV5S | 0 | 11 | CATG | VTISAGNKLTFGGG | Jα17 | 4/9 (44.4%) |
| 9 | CATG | RGRTDKLTFGDG | Jα27 | 2/9 (22.2%) | |||
| 10 | CATG | PDSGYSKLTFGKG | Jα11 | 2/9 (22.2%) | |||
| 8 | CATG | STANKLVFGPG | Jα32 | 1/9 (11.1%) | |||
| AV8-3S | 0 | 11 | CALS | DLGAGYGKLTFGQG | Jα52 | 3/10 (30%) | |
| 10 | CAVS | PMYGAQKLVFGRG | Jα16 | 3/10 (30%) | |||
| 11 | CAVS | HSSGSGDKLTFGSG | Jα46 | 2/10 (20%) | |||
| Others | 2/10 (20%) | ||||||
| AV8-4S | 0 | 9 | CALS | DNAGKAFTFGGG | Jα39 | 5/9 (55.6%) | |
| 9 | CALS | GGSQGKLTFGKG | Jα42 | 1/9 (11.1%) | |||
| 9 | CALS | APSYNKLMFGQG | Jα50 | 1/9 (11.1%) | |||
| 9 | CAVS | GSTANKLVFGPG | Jα32 | 1/9 (11.1%) | |||
| 9 | CALS | GNYGNKVIFGPG | Jα47 | 1/9 (11.1%) | |||
| AV14 | 0 | 11 | CALS | EPGNAGKAFTFGGG | Jα39 | 5/15 (33.3%) | |
| 11 | CALS | EPGNACKAFTFGGG | Jα39 | 2/15 (13.3%) | |||
| 11 | CALS | EERGSQGKLTFGKG | Jα42 | 2/15 (13.3%) | |||
| Others | 6/15 (40%) |
TRAV and TRAJ are assigned under the classification by IMGT website (http://www.imgt.org/). Numbers in parentheses are the frequency of this clone in the total cloned sequences.