Table 3.
The CDR3 amino acid sequences of clonal TCR-β chain in CD4+ T cells in three Hezuo miniature pigs immunised with C-strain vaccine.
| Case | TCR BV gene family | Peak | Size (aa) | 3′ Vβ | N + Dβ + 5′ proximal Jβ | Jβ | Frequency |
|---|---|---|---|---|---|---|---|
| H2 | BV6S | −3 | 11 | CVVR | TLRGNAAQLYFGDG | Jβ2.2 | 7/11 (64%) |
| Others | 4/11 (36%) | ||||||
| H3 | BV30 | −1 | 12 | CAWS | LAGQGGHQTQYFGPG | Jβ2.5 | 4/10 (40%) |
| 11 | CAWS | LEGRGGAEQHFGPG | Jβ2.1 | 3/10 (30%0 | |||
| 11 | CASS | FMGGGPQTQYFGPG | Jβ2.5 | 1/10 (10%) | |||
| 13 | CAWS | LRGTAASYAEQHFGPG | Jβ2.1 | 1/10 (10%) | |||
| 14 | CAWS | RMSYGGGLTQTQYFGPG | Jβ2.5 | 1/10 (10%) | |||
| H4 | BV4S | +3 | 13 | CASS | LGQGGAMTDPLYFGEG | Jβ2.3 | 3/10 (30%) |
| 13 | CASS | VGQGGAMTDPLYFGEG | Jβ2.3 | 2/10 (20%) | |||
| 13 | CASS | SSHGGAVPDPLYFGEG | Jβ2.3 | 2/10 (10%) | |||
| Others | 3/10 (30%) | ||||||
| BV7S | 0 | 10 | CASS | DWDRGKTQYFGPG | Jβ2.5 | 3/11 (27.3%) | |
| 11 | CASS | EAGGLSQTQYFGPG | Jβ2.5 | 3/11 (27.3%) | |||
| 10 | CASS | ITGSDQAQHFGDG | Jβ1.5 | 2/11 (18.2%) | |||
| Others | 3/11 (27.3%) | ||||||
| BV15S | −3 | 8 | CASS | SGTIDYNFGPG | Jβ1.2 | 3/9 (33.3%) | |
| 8 | CASS | RGSNTQHFGPG | Jβ2.4 | 2/9 (22.2%) | |||
| Others | 4/9 (44.4%) |
TRBV and TRBJ are assigned according to the reported sequences on GeneBank database. Numbers in parentheses are the frequency of this clone in the total cloned sequences.