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. 2018 Jan 26;8:1678. doi: 10.1038/s41598-018-19806-z

Figure 3.

Figure 3

MiR-29b preferentially controls Tet3 expression level. (a) Level of Tet1, Tet2, and Tet3 in N2a cells after transfection with miR-29b mimic or control measured by RT-qPCR. *p < 0.05, ***p < 0.001 determined by unpaired t test. (b) Level of Tet1, Tet2, and Tet3 in N2a cells after transfection with miR-29b antagomir or control measured by RT-qPCR. *p < 0.05, **p < 0.01 determined by unpaired t test. (c) Analysis of Tet3 luciferase reporters in the presence of miR-29b mimic, seed mutant miR-29b mimic or control (40 nM) in N2a cells. ***p < 0.001 determined by one-way ANOVA followed by Tukey’s post-hoc test. (d) Analysis of Tet3 3′UTR luciferase reporter and seed sequence mutated Tet3 3′UTR luciferase reporter in the presence of miR-29b mimic. ***p < 0.001 determined by unpaired t test. (e) Analysis of Tet1, Tet2 and Tet3 luciferase reporters in the presence of graded concentrations of miR-29b mimic or control in N2a cells. *p < 0.05, **p < 0.01, ***p < 0.001 determined by unpaired t test. Data represent mean ± s.e.m.