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. 2018 Jan 26;8:1653. doi: 10.1038/s41598-018-19661-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene and protein expression in different organs. (a) PCR analysis of the organ profile of the COX-1-10aa-PGIS gene in CP-Tg mice. PCR was performed using the different organs of CP-Tg mice. An arrow indicates the 445-bp COX-1-10aa-PGIS. Lanes: 1) positive control, 2) negative control, 3) lung, 4) adipose tissue, 5) intestine, 6) brain, 7) heart, 8) liver, 9) pancreas, 10) wild-type tail, 11) uterus, 12) TG tail. (b) Western blot analysis of the different organs of the transgenic mice. The arrow indicates COX-1-10aa-PGIS protein expression at 130 kDa. Lanes: 1) lung, 2) heart, 3) intestine, 4) pancreas, 5) adipose tissue, 6) brain, 7) uterus and 8) liver. The blot was chopped to increase visualization, and the initial blot is attached as Supplementary Fig. 2. (c–e) LC/MS analysis of AA metabolites, prostanoids in urine of the CT-Tg and wild-type mice. (c) Ratio of 6-keto-PGF_1α to PGE₂ in both TG and WT mice. (d) Ratio of 6-keto-PGF_1α to TXB₂ in both CP-Tg and WT mice. The above graph represents the mean ± SD (p < 0.001). (e) Amount of 6-keto-PGF_1α, TXB₂, and PGE₂ in 24 h urine. The values are represented as mean ± SD.