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. 2018 Jan 26;8:1696. doi: 10.1038/s41598-018-19879-w

Figure 3.

Figure 3

CSO is a potent inducer of mϕheal macrophage polarization while attenuating M1 phenotype in acute wounds. (A) Day 7 wound mϕ were harvested from PVA sponges coated with CSO (300 mg) implanted subcutaneously in C57bl/6 mice. Total RNA was isolated and mRNA expression of Arginase-1, CD206, YM1 and VEGF was measured using RTPCR. CSO, solid bars; control, blank bars. (B) Total protein isolated from day 7 wound mϕ of C57bl/6 mice, treated with CSO in vivo, was subjected to capillary electrophoresis immunoassay to measure expression of Arginase-1 protein. (C) Arginase activity of in vivo CSO treated (300 mg) day 7 wound mϕ of C57bl/6 mice were measured by Arginase activity assay kit (Colorimetric). (D) mRNA expression of CD74 was measured in day 7 wound mϕ of C57bl/6 mice treated with CSO (300 mg). (E) PMA-induced superoxide anion production in day 3 wound mϕ of C57bl/6 mice treated in vivo with CSO (300 mg) was measured. For all figure parts, data are expressed as mean ± SEM (n = 3–6); *p < 0.05 compared to wound mϕ treated with equal amounts of white petrolatum (control).