Table 2.
Calculated collision cross sections (in Å2) in N2 and He drift gas for each of two protonation states, P3 and P6, of ten PA-glycans. The experimental values in the presence of N2 drift gas are also listed. The percentage difference between the experimental and calculated CCS in N2 drift gas (%diff = CCScalc − CCSexp/CCSexp × 100%) are given in parenthesis.
| Experiment | Calculated CCS in N2 gas | Calculated CCS in He gas | |||
|---|---|---|---|---|---|
| P3 | P6 | P3 | P6 | ||
| G0F-GN(3)a | 371 | 400 (7.8) | — | 279 | — |
| G0F-GN(6) | 388 | — | 427 (10.1) | — | 296 |
| G0 | 402 | 425 (5.7) | 420 (4.5) | 298 | 292 |
| G0F | 431 | 464 (7.7) | 447 (3.7) | 328 | 315 |
| G1(3) | 391 | 423 (8.2) | 436 (11.5) | 297 | 309 |
| G1(6) | 428 | 444 (3.7) | 450 (5.1) | 313 | 318 |
| G1F(3) | 425 | 454 (6.8) | 476 (12.0) | 322 | 338 |
| G1F(6) | 440 | 475 (8.2) | 467 (6.4) | 340 | 336 |
| G2 | 417 | 477 (14.4) | 455 (9.1) | 340 | 325 |
| G2F | 442 | 475 (7.5) | 481 (8.8) | 342 | 350 |
aThe core GlcNAc, rather than that of the α1-3 branch, was protonated.