Table 2.

Accuracy and precision (% RSD) of HCQ, DCQ, BDCQ and DHCQ in mouse blood and tissues.

Bio Matrix	Analytes	LLOQ (1 ng/mL)		LQC (6 ng/mL)		MQC (200 ng/mL)		HQC (1500 ng/mL)
		Accuracy	% RSD	Accuracy	% RSD	Accuracy	% RSD	Accuracy	% RSD
Blood	HCQ	112.5	5.7	110.1	6.1	98.3	8.4	112.5	6.6
	DCQ	102.3	2.5	107.2	4.2	97.8	8.2	102.3	3.2
	BDCQ	104.6	3.4	108.4	3.7	96.9	5.4	105.6	4.8
	DHCQ	100.7	7.5	112.7	1.3	96.7	1.3	100.7	3.8
Liver	HCQ	99.2	5.6	98.1	3.7	97.8	10	87.3	1.8
	DCQ	100.7	6	112.7	4.3	96.7	1.3	105.3	5.8
	BDCQ	104.6	1.4	108.4	3.7	96.9	5.4	107.5	4.8
	DHCQ	104.5	1.4	114.5	3.3	87.1	5.6	91.1	3.2
Lungs	HCQ	90.5	5.5	114.7	7.8	92.1	10.8	84.7	4.3
	DCQ	109.9	2.9	100.4	0.9	103.1	10.3	107	5.4
	BDCQ	112.5	11.9	112.1	7.1	96.3	12.4	102.8	6.6
	DHCQ	102.3	2.5	107.2	2.2	97.8	11.2	90.2	3.2
Kidney	HCQ	102.9	14.3	106.7	2.3	105.8	10.2	107.3	1.4
	DCQ	112.2	10.6	103.3	2.3	103.9	9.1	108.3	4.5
	BDCQ	100.2	4.3	114.4	3.1	100.6	9.1	101.1	55
	DHCQ	101.3	2.5	107.5	5.8	101.9	11.2	98.3	2.7
Spleen	HCQ	90.9	5.1	95.5	4.6	107.6	9.2	107.7	6.1
	DCQ	109.6	7.1	105.3	2.7	90.1	11.7	106.5	4.6
	BDCQ	104.3	4.3	109.9	9.7	104.7	5.4	107.8	8.9
	DHCQ	90.1	7.1	106.1	2.9	101.5	9.2	109.1	8.9
Heart	HCQ	116.6	4.2	105.7	2.4	101.4	10.2	108.7	7.1
	DCQ	110.8	3.8	99.9	4.7	102.5	9.1	95.7	5.5
	BDCQ	103.2	5.1	115.1	3.2	92.2	10	108.1	7.2
	DHCQ	101.7	4.4	109.6	5.4	100.5	7.4	90.5	4.7