Figure 5.

Ca²⁺ and reducing agents modulate ischemia and reperfusion-induced depolarization. (a) Representative membrane potential recording in cortical pyramidal neurons (DIV 14–21) treated with 5 mM NaN₃ +AET in the absence of extracellular Ca²⁺. Right panel shows the membrane potential values at 0 and 15 min post NaN₃+AET for each experiment (n = 5). (b) Representative membrane potential recording in cortical pyramidal neurons (DIV 14–21) treated with 5 mM NaN₃+AET and 1 mM NAC. Right panel shows the membrane potential values at 0 and 15 min post NaN₃+AET and 1 mm NAC for each experiment (n = 5). (c) Representative current recording showing the effect of 5 mM NaN₃+AET in the absence of extracellular Ca²⁺. Right panel shows the current values at 0 and 15 min post NaN₃+AET for each experiment (n = 5). (d) Representative current trace showing the effect of 5 mM NaN₃+AET and 1 mM NAC. Right panel shows the current values at 0 and 15 min post NaN₃+AET and 1 mm NAC for each experiment (n = 6). Black arrows indicate t = 0 and t = 15, respectively. (e) Current time course measured at 100 mV from a voltage ramp as described in Methods in cortical pyramidal neurons exposed to 30 min OGD in the presence of AET (OGD+AET) and during reperfusion (Reperfusion+AET). (f) Mean current values during Reperfusion+AET and upon exposure to 100 µM glibenclamide (Glib+AET, n = 5).