Skip to main content
. 2017 Dec 12;11(6):636–647. doi: 10.1080/19336950.2017.1393131

Figure 3.

Figure 3.

hKir6.2(A28V) has a dominant-negative effect on KATP channel surface expression in HEK cells. (A) An HA-tag was inserted into the extracellular domain of rSUR1 and then co-transfected with one of the hKir6.2 tandem constructs that are linked by a self-cleaving P2A linker. Only cells transfected with the wt-P2A-wt hKir6.2 construct showed strong surface staining (top row). Surface expression was not apparent in HEK cells expressing KATP channels that contain wt-P2A-A28V, A28V-P2A-wt or A28V-P2A-A28V hKir6.2. (B) Quantitative analysis of the surface signal from cells transfected with various hKir6.2 tandem constructs. KATP channels formed from wt-P2A-wt tandem construct expression had much stronger surface staining signals compared to any other hKir6.2 tandem constructs that contain the A28V Kir6.2 mutation. (**p = 0.001, ***p < 0.0005, one-way ANOVA with posthoc multiple comparison test, n = 8 for wt-P2A-wt, 8 for A28V-P2A-A28V, 9 for A28V-P2A-wt, and 14 for wt-P2A-A28V).