Table 1.

Variance (Var) in Gene Expression Compared Between the Two Lines of K. marmoratus (R and DAN) and Treatments (Infection and Control) for Each One of Nine Immune-Related Target Genes, Analyzed Using RT-qPCR

	MHC I	IRGF1	CD4-1	FGG	C7	MHC II	AHSAb1	CXCL11.8	LECT2
Var line DAN	0.9531	0.1729	0.0003	2.6146	0.0158	0.0019	0.0005	0.0337	0.0007
Var line R	0.7725	0.0000	0.0012	3.9938	0.0914	0.0132	0.0016	0.0023	0.0000
P (between lines)	0.072	3.321e−07	0.012	0.627	0.057	8.61e−05	0.038	0.018	0.008
Var Control	0.2302	0.0418	0.0002	0.4798	0.0052	0.0059	0.0002	0.0003	0.0001
Var Infection	1.2466	0.1306	0.0010	4.2703	0.0755	0.0095	0.0014	0.0279	0.0006
P (between treatments)	0.096	0.109	0.154	0.011	0.102	0.038	0.769	0.024	0.447

Note.—Significant differences in italics (P < 0.05). The target genes chosen serve important immune functions including antigen presentation (MHC I-uka, MHC II-dab, CD4-1), membrane regulation and attack (C7 and IRGF1), clot formation (FGG), immune regulation and cell trafficking (LECT2 and CXCL11.8) and the ATPase activity of heat shock proteins (AHSAb1). Variances between groups were compared using the Fligner-Killeen test of homogeneity of variances (Conover et al. 1981).