Fig. 1.

Possible order of investigation for rationally targeted molecular testing based on tumor location and histology. Upfront next-generation sequencing (DNA + RNA) can also be used to supersede this order and give the most comprehensive overview. PA, pilocytic astrocytoma; (A)GG, (anaplastic) ganglioglioma; PXA, pleomorphic xanthoastrocytoma; DNET, dysembryoplastic neuroepithelial tumor; O, oligodendroglioma; OA, oligoastrocytoma; RGNT, rosette-forming glioneuronal tumor; AG, angiocentric glioma; DA (pediatric-type) diffuse glioma; mut, mutation; dup, tyrosine kinase internal tandem duplication; fus, fusion.