Skip to main content
. Author manuscript; available in PMC: 2018 Oct 13.
Published in final edited form as: Sci Immunol. 2017 Oct 13;2(16):eaan6049. doi: 10.1126/sciimmunol.aan6049

Figure 3. Core 2 O-glycan synthesis in memory CD8+ T cell subsets.

Figure 3

(A) Memory P14 CD8+ T cells were analyzed 75 days post-LCMV infection for expression of CD62L and KLRG1. (B-E) Memory P14 CD8+ T cells subsets from (A) were analyzed for expression of (B) core 2 O-glycans (1B11), (C) CD122, (D) E-selectin binding, and (E) P-selectin binding. (F) 1B11- cells from each subset as shown in (A) were sorted by FACS and cultured in vitro for 3 days in media alone or supplemented with 50 or 250 ng/ml of IL-15. (G) Memory CD8+ T cell subsets were sorted and 5 × 105 were transferred into B6 mice and challenged with CpG intranasally. Recruitment into the challenged lung and binding of E- and P-selectin was analyzed at 60 hours post-challenge. (H-J) Quantification of data shown in (G)