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. Author manuscript; available in PMC: 2018 Jan 26.
Published in final edited form as: Cell Rep. 2018 Jan 2;22(1):17–26. doi: 10.1016/j.celrep.2017.12.031

Figure 3. Global Interrogation of eIF5B-Dependent Targets.

Figure 3

(A) Workflow of RNomic and proteomic analyses.

(B) RNomic analysis of translation efficiency (TE) and steady-state RNA levels. Transcripts exhibiting decreased TE (≤0.5-fold) are highlighted in red.

(C) eIF5B-dependent targets that exhibit decreased TE, protein output, and steady-state protein levels (≤0.75-fold).

(D) Agreement between TE, steady-state RNA level and protein output.

(E) Effect of eIF5B depletion on magnitude of TE decrease across hypoxia-responsive mRNA classes.

(F) Proportions of the three hypoxia-responsive mRNA classes under eIF5B-replete and eIF5B-depleted (by siRNA) conditions.

See also Figure S3.

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