Table 1. Primers used to amplify and clone APP III gene sequences and E. coli ClyA sequences.
Gene | Primer sequences (5’-3’) |
---|---|
ClyA-F | CGCCGAGCTCATGACTGAAATCGTTGCAGATA |
ClyA-R | TGCTCTAGAGACTTCAGGTACCTCAAAGAGTG |
ApxIAr-F | TGCTCTAGATATGCGGGTAACGGACATGATG |
ApxIAr-R | ACGCGTCGACAATATCCTTACCTAAACCACCGTAG |
ApxIIAr-F | ACGCGTCGACAATTTAGGTGCTGGTAACGATAATG |
ApxIIAr-R | TGCCAGTTTATCTTGTCCGC |
ApxIIIAr-F | CTCTGGCGGACAAGATAAACTGGCACATCTAGGCAATGGTAATGA |
ApxIIIAr-R | CCCCAAGCTTTTAGTGGTGGTGGTGGTGGTGAAAATCGCTACCATCGCCTCCT |
The underline portions of the primer sequences were the restriction enzyme cutting sites (ClyA-F (forward): SacI; ClyA-R (reverse): XbaI; ApxIAr-F: XbaI; ApxIAr-R: SalI; ApxIIAr-F: SalI; ApxIIIAr-R: HindIII).