Table 1. Primers used to amplify and clone APP III gene sequences and E. coli ClyA sequences.

Gene	Primer sequences (5’-3’)
ClyA-F	CGCCGAGCTCATGACTGAAATCGTTGCAGATA
ClyA-R	TGCTCTAGAGACTTCAGGTACCTCAAAGAGTG
ApxIAr-F	TGCTCTAGATATGCGGGTAACGGACATGATG
ApxIAr-R	ACGCGTCGACAATATCCTTACCTAAACCACCGTAG
ApxIIAr-F	ACGCGTCGACAATTTAGGTGCTGGTAACGATAATG
ApxIIAr-R	TGCCAGTTTATCTTGTCCGC
ApxIIIAr-F	CTCTGGCGGACAAGATAAACTGGCACATCTAGGCAATGGTAATGA
ApxIIIAr-R	CCCCAAGCTTTTAGTGGTGGTGGTGGTGGTGAAAATCGCTACCATCGCCTCCT

The underline portions of the primer sequences were the restriction enzyme cutting sites (ClyA-F (forward): SacI; ClyA-R (reverse): XbaI; ApxIAr-F: XbaI; ApxIAr-R: SalI; ApxIIAr-F: SalI; ApxIIIAr-R: HindIII).