Fig 5. Effects of c-Jun siRNA on IL-33-induced IL-8 secretion from HUVECs.

Antibody-free HUVECs were transfected with control scrambled siRNA or c-Jun siRNA (100 nmol/L) for 48 hrs and then stimulated with or without IL-33 (10⁻⁹ mol/L) for 24hrs. (A) Cell lysates were evaluated for knock down of c-Jun by Western immunoblot analysis. β-Actin was used for loading control. (B) IL-8 concentration in the supernatant as measured by ELISA (n = 6). Bars represent IL-8 protein secretion per 10 ⁵ cells. *P <0.05 vs. untreated control. †P <0.05 vs. scrambled siRNA, ‡P <0.05 vs. scrambled siRNA plus IL-33.